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Cellular Neurophysiology


High-resolution electrophysiology

 

Whole-cell, perforated patch, cell-attached, voltage-clamp, current-clamp, membrane capacitance, carbon fiber amperometry, flash photolysis, loose-patch, field potential recordings

Electron microscopy

 

cryo-EM, REM, CLEM (correlative light and electron microscopy)

Transgenic mouse models

CRISPR/Cas; global or tissue-specific Knock-in and Knock-out

Collaborative studies include: in situ hybridization, single cell/small tissue isolation and gene analysis

 

Cell culture

Neurons from various brain regions, chromaffin cells, cytotoxic T lymphocytes, astrocytes, BHK cells, 293 cells and many more 

 

Flow cytometry

FACS AriaIII, 11 colors, sorting and analyses 

 

in vivo microscopy

2-photon in vivo microscopy of graft rejection inside the anterior chamber of the eye

High-resolution live cell and tissue imaging

Calcium imaging, stimulated emission depletion (STED) microscopy, total internal reflection fluorescence microscopy (TIRFM), particle tracking, confocal microscopy (LSM780), structured illumination microscopy (SIM), single molecule fluorescence (PALM, STORM)