Lab Members AG Rettig
Lab members
Research
Techniques
Funding
Publications
Research
Our research is directed towards elucidating the molecular mechanisms underlying synaptic transmission in neurons, neuroendocrine cells and immune cells.
The ultimate goal is to understand how communication between these cells is mediated and modulated.
In order to achieve these goals we are using a combination of high-resolution electrophysiological and imaging methods on defined genetic model systems.
Extrapolation of that knowledge from individual synapses to networks of the brain and immune system will enable us to understand the function of these networks and identify potential strategies for treatment of disorders.
High-resolution electrophysiology
Whole-cell, perforated patch, cell-attached, voltage-clamp, current-clamp, membrane capacitance, carbon fiber amperometry, flash photolysis, loose-patch, field potential recordings
High-resolution live cell and tissue imaging
Calcium imaging, STED (stimulated emission depletion) microscopy, total internal reflection fluorescence microscopy (TIRFM), particle tracking, confocal microscopy (Elyra), structured illumination microscopy (SIM), photoactivatable light microscopy (PALM)
Tissue culture
Neurons from various brain regions, chromaffin cells, cytotoxic T lymphocytes, astrocytes, BHK cells, 293 cells and many more
Transgenic mouse models
CRISPR/Cas; Tissue-specific; Inducible; Knock-in; Molecular biology
Collaborative studies include: in situ hybridization, single cell/small tissue isolation and gene analysis
Electron microscopy
chemical fixation EM, high pressure freezing EM, correlative light and electron microscopy (CLEM)
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FACS
FACS AriaIII
Four laser lines
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2017
Chang HF, Bzeih H, Chitirala P, Ravichandran K, Sleiman M, Krause E, Hahn U, Pattu V, Rettig J. Preparing the lethal hit: interplay between exo- and endocytic pathways in cytotoxic T lymphocytes. Cell Mol Life Sci. 2017 Feb;74(3):399-408. doi: 10.1007/s00018-016-2350-7. PMID: 27585956
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2017
Sleiman M, Stevens DR, Rettig J. Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses. Methods Mol Biol. 2017;1584:157-169. doi: 10.1007/978-1-4939-6881-7_11. PMID: 28255702
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2016
Chang HF, Bzeih H, Schirra C, Chitirala P, Halimani M, Cordat E, Krause E, Rettig J, Pattu V. Endocytosis of Cytotoxic Granules Is Essential for Multiple Killing of Target Cells by T Lymphocytes. J Immunol. 2016 Sep 15;197(6):2473-84. doi: 10.4049/jimmunol.1600828. PMID: 27527597
Bhat SS, Friedmann KS, Knörck A, Hoxha C, Leidinger P, Backes C, Meese E, Keller A, Rettig J, Hoth M, Qu B, Schwarz EC. Syntaxin 8 is required for efficient lytic granule trafficking in cytotoxic T lymphocytes. Biochim Biophys Acta. 2016 Jul;1863(7 Pt A):1653-64. doi: 10.1016/j.bbamcr.2016.04.014. PMID: 27094127
Thorn P, Zorec R, Rettig J, Keating DJ. Exocytosis in non-neuronal cells. J Neurochem. 2016 Jun;137(6):849-59. doi: 10.1111/jnc.13602. PMID: 26938142
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2015
Man KN, Imig C, Walter AM, Pinheiro PS, Stevens DR, Rettig J, Sørensen JB, Cooper BH, Brose N, Wojcik SM. Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis. Elife. 2015 Nov 17;4. pii: e10635. doi: 10.7554/eLife.10635. PMID: 26575293
Stevens DR, Ratai O, Rettig J. Interactions with membrane lipids and SNARE complex underlie dual roles for Calcium-dependent activator proteins for secretion (CAPS) in large dense-core vesicles (LDCVS) priming. Physiological Mini Reviews. 2015 Nov-Dec Vol. 8 Nº 6.
Ming M, Schirra C, Becherer U, Stevens DR, Rettig J. Behavior and Properties of Mature Lytic Granules at the Immunological Synapse of Human Cytotoxic T Lymphocytes. PLoS One. 2015 Aug 21;10(8):e0135994. doi: 10.1371/journal.pone.0135994. PMID: 26296096
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2014
Nguyen Truong CQ, Nestvogel D, Ratai O, Schirra C, Stevens DR, Brose N, Rhee J, Rettig J. Secretory vesicle priming by CAPS is independent of its SNARE-binding MUN domain. Cell Rep. 2014 Nov 6;9(3):902-9. doi: 10.1016/j.celrep.2014.09.050. PMID: 25437547
Koers EJ, van der Cruijsen EA, Rosay M, Weingarth M, Prokofyev A, Sauvée C, Ouari O, van der Zwan J, Pongs O, Tordo P, Maas WE, Baldus M. NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field. J Biomol NMR. 2014 Nov;60(2-3):157-68. doi: 10.1007/s10858-014-9865-8. PMID: 25284462
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2013
Pattu V, Halimani M, Ming M, Schirra C, Hahn U, Bzeih H, Chang HF, Weins L, Krause E, Rettig J. In the crosshairs: Visualizing lytic granules by high resolution microscopy and electrophysiology. Front Immunol. 2013 Nov 27;4:411. doi: 10.3389/fimmu.2013.00411. PMID: 24348478
Bost A, Pasche M, Schirra C, Becherer U. Super-resolution microscopy in studying neuroendocrine cell function. Front Neurosci. 2013 Nov 25;7:222. doi: 10.3389/fnins.2013.00222. PMID: 24324394
Hugo S, Dembla E, Halimani M, Matti U, Rettig J, Becherer U. Deciphering dead-end docking of large dense core vesicles in bovine chromaffin cells. J Neurosci. 2013 Oct 23;33(43):17123-37. doi: 10.1523/JNEUROSCI.1589-13.2013. PMID: 24155316
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2012-1991
2012
Schattling B, Steinbach K, Thies E, Kruse M, Menigoz A, Ufer F, Flockerzi V, Brück W, Pongs O, Vennekens R, Kneussel M, Freichel M, Merkler D, Friese MA. TRPM4 cation channel mediates axonal and neuronal degeneration in experimental autoimmune encephalomyelitis and multiple sclerosis. Nat Med. 2012 Dec;18(12):1805-11. doi: 10.1038/nm.3015. PMID: 23160238
Becherer U, Medart MR, Schirra C, Krause E, Stevens D, Rettig J. Regulated exocytosis in chromaffin cells and cytotoxic T lymphocytes: how similar are they? Cell Calcium. 2012 Sep-Oct;52(3-4):303-12. doi: 10.1016/j.ceca.2012.04.002. PMID: 22560267
Pasche M, Matti U, Hof D, Rettig J, Becherer U. Docking of LDCVs is modulated by lower intracellular [Ca2+] than priming. PLoS One. 2012;7(5):e36416. doi: 10.1371/journal.pone.0036416. PMID: 22590540
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